My cell line U87MG is getting detached after splitting, what could be the reason?

Few weeks back there was fungal infection in some of my petri dishes. So, I thoroughly cleaned the incubator using bleach and rectified alcohol, revived fresh stocks, prepared everything fresh and...

07 August 2015 2,580 17 View

How do we calculate scale for microscopic images and how do we add a scale bar to it?

I need to put scale bar in my microscopic images at 100x, 40x, 20x and 10x

04 May 2015 9,390 1 View

Can anyone help me please in calculating the IC50 dose of the drug using the Graph Prism software?

I have followed the tutorial still it is not helpful! please help

02 March 2015 6,915 2 View

Can we perform micronucleus assay without using cytochalasin B?

Since it is a very hazardous chemical I do not want to use it and also it is expensive too.

06 July 2014 1,282 17 View

please anyone give me a good and sensitive protocol for DNA fragmentation to check apoptosis in cancer cell lines

I am not able to repeat results with the previous protocol

04 May 2014 2,341 5 View

Can we perform flow cytometry on -80 frozen samples?

We collected the plants 2 months back and stored them in -80

03 April 2014 9,013 5 View

I require caspase antibodies, can anyone help?

I require the caspases for western blotting.

02 March 2014 9,439 4 View

Can anyone suggest me a good lab in New Delhi, India where I can perform western blotting and flow cytometry of cancer cells after drug treatment?

I am really frustrated as I cannot perform it in my lab.

01 February 2014 2,089 2 View

How to design primers for EST-SSR Markers?

For differentiating between male and female species of a plant.

01 February 2014 7,055 5 View

How to discard etbr gels?

Is there any chemical methods to treat etbr gels , so as to precipitate etbr or negate its harmful effect

31 December 2013 9,109 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

No title

01 March 2021 3,905 5 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View