Under which filter should I view acridine orange and Etbr staining for checking cell viability of cancer cells?

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Quais Hassan

Blue light (I use the DAPI filter). AO staining from dsDNA (alive cells) should be green, and EtBr staining (from dead, permeabilized cells) should be orange.

Shweta Umar

can i perform Etbr/AO staining to my treated cancer cell cultured on 12 well plate ? anyone please provide a full protocol for staining and detection