I require the caspases for western blotting.
Well, first of all, which caspases do you want to check and in which cell system?
In our lab we used antibodies for caspase3/cleaved caspase3 and caspase8 from cell signal and the bands are very good.
Cell signal, 9661S for Cleaved Caspase 3 in our lab.
I use caspase-3 and 9 cell signalling ...
caspase 3 9662 (for detect pro-caspase 3 e caspase 3 mature)
caspase 9 9504.
I need caspase3, caspase8
Few weeks back there was fungal infection in some of my petri dishes. So, I thoroughly cleaned the incubator using bleach and rectified alcohol, revived fresh stocks, prepared everything fresh and...
07 August 2015 2,754 17 View
I need to put scale bar in my microscopic images at 100x, 40x, 20x and 10x
04 May 2015 9,576 1 View
I have followed the tutorial still it is not helpful! please help
02 March 2015 7,056 2 View
Since it is a very hazardous chemical I do not want to use it and also it is expensive too.
06 July 2014 1,445 17 View
I am not able to repeat results with the previous protocol
04 May 2014 2,471 5 View
We collected the plants 2 months back and stored them in -80
03 April 2014 9,172 5 View
I just want to know the cells should be viewed under which filter.
03 April 2014 706 2 View
I am really frustrated as I cannot perform it in my lab.
01 February 2014 2,229 2 View
For differentiating between male and female species of a plant.
01 February 2014 7,243 5 View
Is there any chemical methods to treat etbr gels , so as to precipitate etbr or negate its harmful effect
31 December 2013 9,233 3 View
"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....
10 August 2024 8,621 1 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...
10 August 2024 8,992 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Who will bear moral responsibility for the deaths of thousands of people in the event of an earthquake? Weeks and months remain before the onset of strong earthquakes that bring death to...
08 August 2024 6,134 12 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View