My cell line U87MG is getting detached after splitting, what could be the reason?

Few weeks back there was fungal infection in some of my petri dishes. So, I thoroughly cleaned the incubator using bleach and rectified alcohol, revived fresh stocks, prepared everything fresh and...

07 August 2015 2,580 17 View

How do we calculate scale for microscopic images and how do we add a scale bar to it?

I need to put scale bar in my microscopic images at 100x, 40x, 20x and 10x

04 May 2015 9,390 1 View

Can anyone help me please in calculating the IC50 dose of the drug using the Graph Prism software?

I have followed the tutorial still it is not helpful! please help

02 March 2015 6,915 2 View

Can we perform micronucleus assay without using cytochalasin B?

Since it is a very hazardous chemical I do not want to use it and also it is expensive too.

06 July 2014 1,282 17 View

please anyone give me a good and sensitive protocol for DNA fragmentation to check apoptosis in cancer cell lines

I am not able to repeat results with the previous protocol

04 May 2014 2,341 5 View

Can we perform flow cytometry on -80 frozen samples?

We collected the plants 2 months back and stored them in -80

03 April 2014 9,013 5 View

Under which filter should I view acridine orange and Etbr staining for checking cell viability of cancer cells?

I just want to know the cells should be viewed under which filter.

03 April 2014 576 2 View

Can anyone suggest me a good lab in New Delhi, India where I can perform western blotting and flow cytometry of cancer cells after drug treatment?

I am really frustrated as I cannot perform it in my lab.

01 February 2014 2,089 2 View

How to design primers for EST-SSR Markers?

For differentiating between male and female species of a plant.

01 February 2014 7,055 5 View

How to discard etbr gels?

Is there any chemical methods to treat etbr gels , so as to precipitate etbr or negate its harmful effect

31 December 2013 9,109 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

No title

02 March 2021 3,060 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View