Amit Kumar

6 Questions 28 Answers 0 Followers

Questions related from Amit Kumar

How to execute MOTHUR in windows?

I have very little knowledge about command line in C. I would like to run MOTHUR for my clone library. I tried to learn using some manuals but I could not succed. Can anyone teach me how to do it...

07 July 2014 8,534 4 View

How many days can RNA be stored in -80 without degradation?

I am afraid that my RNA degrades. I have prepared about 1.5 months back and will send for sequencing in the first week of Jan. In total, about 2 months. I think it wont degrade but I am afraid..it...

12 December 2013 3,019 2 View

What could be the reason that my total RNA on agarose gel is showing only a 28S band?

I am doing a preparation using ctab extraction method followed by RNease Qigen mini elute kit and at the end I am getting a good concentration and ratios of 260/280 and 2860/230, but on agarose...

11 November 2013 1,853 26 View

Can someone answer a question regarding normalization of qPCR?

Any particular gene may or may not expressed similarly in any stress condition. Maybe if tubulin is good for brown algae in salinity stress, its not necessary equally good for pH stress. Should...

07 July 2013 2,763 1 View

Can someone help me regarding band separation with a thick background smear in SDS PAGE?

Though a separate band pattern is visible, there is a huge smear in the lane. The protein is not overloaded. My sample is rich in polyphenols and polysachharides. I have done TCA precipitation and...

06 June 2013 3,072 13 View

Is anyone doing or has done RNA extraction from Sargassum?

I have some of the protocols on brown algae but the yield is always less in my experiments. I am a bit worried that this is only the case for me, or is the yield from brown algae generally less?...

03 March 2013 6,790 8 View