Shailendra Dwivedi

5 Questions 37 Answers 0 Followers

Questions related from Shailendra Dwivedi

What would be best for the normalization of miRNA expression in human samples of whole blood, serum and tissues ?

As few study shown RNU6 B in tissue,while few claimed about miR-16 etc....As I have to perform in all the above samples in serum, whole blood and Tisssue samples.Experts comment needed who are...

05 May 2016 3,110 4 View

How to enrich tumor cells from tissue specimens which contain non cancerous cells?

Pure cancerous cells are needed for the gene quantification after RNA isolation from the cancerous tissue but the contamination is a major problem in biopsy tissues.

08 August 2013 1,052 13 View

What is the best method for isolation and characterization of methylated acetylated gene from the human blood samples?

Is there some kits which are capable to isolate these changed genes like qiagen etc or traditional methods of electrophoresis.

07 July 2013 9,994 4 View

Can someone please suggest online free software for the analysis of relative gene expression after obtaining delta ct values on real time pcr?

It would be more convenient if someone could suggest some excel sheet method/software to calculate delta ct and folds expression. Manually one by one is quite complex and time consuming.

07 July 2013 9,959 6 View

Please Comment on "Molecular biology should be introduced at graduate level in Medical courses like MBBS,BDS"

as several diagnostic test like Hepatitis virus,Viral load is currently diagnosed by the real time pcr and further new variants like H1N1 viruses ,several RNA virus are screen out by the technique.

06 June 2013 9,683 11 View