How to get pure exosomal miRNA population free of contaminats like miRNA bound to lipoprotein, Ago2? or is it not necessary?

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How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

How to determine tannin content in fodder pellets?

22 July 2024 8,163 3 View

Can we find the Gromacs version from XTC file or TRR?

Hello everyone, This soudns like a strange question, but I created five XTC files using five different versions of GROMACS some years ago. All other files are missing, and I want to repeat the...

16 July 2024 7,205 2 View

Kraft lignin and MMA(Methyl meth acrylate) free radical polymerization for synthesis of thermoplastics?

I am conducting free radical polymerization using Kraft lignin and MMA, with DMSO serving as the solvent and there is also an initiator. Following the reaction, I obtain a solution, but I am...

15 July 2024 9,827 1 View

How do the complex dynamics of the oral microbiome interact with the host immune system affect periodontal disease development and treatment response?

This research question explores the complex dynamics of interactions between the oral microbiome and the host immune system, particularly in the development and treatment response of periodontal...

09 July 2024 7,922 1 View

How to pulbish review article in research gate?

Please provide guidance for publishing in research gate

09 July 2024 8,312 2 View

To what extent should "reading comprehension" be analyzed in an A.R. based on phonological awareness instruction to EFL students using a webtool?

Dear Colleagues and academy, I am conducting an action research study to understand the impact of phonological awareness activities using Quizlet and Boom on the reading comprehension skills of...

02 July 2024 8,480 0 View

How to apply an external electric field in gaussian 16 or Material studio DMOL3 ?

I need to apply an external electric field to 4-hydroxybenzoic acid to compare the molecule's reactivity descriptors. Could you guide me on how to apply the electric field using Gaussian and...

01 July 2024 4,192 3 View

How to convert energy count spectrum into 2D/3D image?

Respected researcher, I want to know that is there any way or one can help in generating a 2D/3D image using the energy count spectrum of from backscatter methods. How can I design such an...

29 June 2024 10,023 1 View

Is there any in-vitro method to test the Immunogenicity of a novel Antigen?

I am trying to quantify the release of Nitric oxide to test the Immunogenicity of my antigen on U937 cells (Monocytes). Based on the literature, I am supposed to convert 1x105 cells/ml to...

27 June 2024 3,131 2 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

I found the zeta potential of exosomes in DLS and it was -8mv. So is it correct or not ?

What should be the zeta potential of bovine milk exosomes

25 July 2024 7,376 5 View

Can I use sonicator only to lyse the exosomes and measure their protein contents using BCA assay kit?

Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...

23 July 2024 4,603 4 View

About exosome isolation?

Hi, All, I am relatively new for exosome isolation. I used an izon column 70nm to isolate exosomes from differentiated neural cells, but the concentrations were pretty low after NTA analysis (I...

01 July 2024 2,558 0 View

Which modeling software and force field suitable for miRNA-mRNA molecular simulation?

Hello everyone, I am currently working on miRNA- mRNA interaction study. Further, I would like to do MD simulations for the miRNA-mRNA models. For the Simulation, I have modelled this complex...

24 June 2024 3,507 2 View

Does anybody know of a cell type with enriched expression of CD63, CD9 and CD81?

I need an appropriate positive control for WB antibodies I am using to confirm the presence of exosomes in samples. The thing is I am having reproducibility issues, and I would like distinguish...

19 June 2024 4,210 3 View

Which is the best small Extracellulare Vesicles (sEVs) concentration to obtain enough RNA for miRNA analysis?

Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...

18 June 2024 2,799 4 View

How can I optimize the protocol of pirfenidone loading into the exosomes (sonication or electroporation)?

What parameters are important in this process?

17 June 2024 4,463 0 View

What is the best way to synthesize cDNA of miRNA; using poly A kit or stem loop PCR?

Recently, I have been trying to quantify the expression of miRNA. I have extracted the miRNA. However, I am not clear about using the cDNA synthesis kit usually there are two kits providing by...

16 June 2024 9,723 2 View

Extraction RNA with miRVANA kit?

Hello everyone, I would like to know if any of you ever tried to assess the yeld of RNA extraction with the mirVana™ miRNA Isolation Kit, without phenol from ThermoFisher. I am experincing some...

06 June 2024 5,715 4 View