What parameters are important in this process?
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View
I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?
29 July 2024 3,229 1 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
What should be the zeta potential of bovine milk exosomes
25 July 2024 7,376 5 View
Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...
23 July 2024 4,603 4 View
I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...
15 July 2024 2,414 1 View
Hi, All, I am relatively new for exosome isolation. I used an izon column 70nm to isolate exosomes from differentiated neural cells, but the concentrations were pretty low after NTA analysis (I...
01 July 2024 2,558 0 View
Hello I have prepared metal nanoparticles and did zeta potential. Normally there is a single peak in sample but now there are 3 peaks. I prepared sample for analysis first I prepared 10 mg/mL...
30 June 2024 1,063 4 View
Hey all what are the ways by which an encapsulated drug inside BSA may escape from its encapsulation. We had synthesised curcumin encapsulated BSA by desolvation method followed by centrifugation...
23 June 2024 8,785 5 View
I have truck tire particles that I am using in my column experiment. I am trying to do size determination using the Malvern Zetasizer. My sample concentration is 0.1 mg/L, i.e., 10 g of sample was...
22 June 2024 8,857 6 View
