I need an appropriate positive control for WB antibodies I am using to confirm the presence of exosomes in samples. The thing is I am having reproducibility issues, and I would like distinguish whether it is my sample that is the problem because it has no exosomes or if it is the antibodies that aren't working properly. Running a cell lysate of a cell known to express these markers would help me determine this.
Thanks to anyone who took the time to read this and reply.
Hello Krish Dodani,
You may use the colon adenocarcinoma cell line (SW480 cells) as a positive control for exosomal markers. Though CD81 expression is low in SW480, it shows high expression of CD63 and CD9 markers. See Fig 1A in the below attached article.
https://www.clinicaltherapeutics.com/article/S0149-2918(14)00267-7/pdf
Best.
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