Ankit Pal

4 Questions 10 Answers 0 Followers

Questions related from Ankit Pal

How to check miRNA transfection efficiency in case of buffalo endometrial epithelial cells?

I am trying to transfect miRNA into buffalo endometrium epithelium cells with Lipofectamine RNAiMAX. To check miRNA transfection efficiency manufacturer's protocol has suggested using a KIF11...

13 October 2021 5,395 2 View

Which Research institute in India has Nanoparticle tracking Assay facility?

I need to validate extracellular vesicle isolated from seminal plasma

04 November 2019 1,401 4 View

Why exosomal miRNA concentarion fluctates so much among the blood plasma samples?

I am isolating Exosomal miRNA from the blood plasma of buffalo. But miRNA concentration always varies from sample to sample. Sometimes I get high as 40ng/microL but sometimes as low as 3ng/microL...

01 January 1970 3,441 6 View

How to get pure exosomal miRNA population free of contaminats like miRNA bound to lipoprotein, Ago2? or is it not necessary?

I am isolating exosomal miRNA using precipitation based kits. but i think these kits also isolate contaminats also like microvesicles, miRNA bound with AGO2 protein and lipoproteins. How to...

01 January 1970 5,448 3 View