How to get fungal colony from solid media for extraction of RNA?

More Ayaz Farzand's questions See All

Why the expression of control in qRT-PCR analysis always equal to 1?

i have been doing qPCR analysis for some Pathogenicity related genes in Fungus but when i start calculating the fold changes using given protocol. the expression of control become 1 always. can...

05 June 2019 1,290 9 View

How to predict plant growth promoting genes in the genome of Bacillus strains?

i wanted to study the genomic basis of Plant growth promotion in the genome of bacteria. Is there any software or website which can predict the genes involved directly or indirectly in promotion...

03 April 2019 8,989 4 View

Can hydrocholoric acid be used as a replacement of Picric acid?

I wanted to test the ability of bacteria to produce Hydrogen cyanide. In the procedure, different scientist have used Picric acid but we can't use this acid in our lab so i want to know if HCL can...

03 April 2019 6,415 1 View

Is it possible to detect Bacilysin by MALDI-TOF/MS in culture filtrates of Bacillus strains?

i am trying to detect bacilysin by MALDI-TOF/MS in the cultural filtrates of bacillus strains but i am unable to detect it. is there any reason for not detecting bacilysin in crude cultural...

31 December 2018 1,728 3 View

Do lipopeptides effect pathogenecity genes of a fungus during direct antagonism?

i have been working on mechanism by which bacillus strains can fight with fungal pathogens when they come in direct contact to each other. it has been well documented that antibiosis plays an...

31 December 2018 2,002 3 View

I have a problem with restriction digestion ?

i have been doing restriction digestion but after digestion when i run the gel, the band is there but not smooth as in control. what can be the problem with this kind or band?

06 July 2018 2,438 2 View

If there any specific protocol for extracting fengycin only ?

i wanted to get Fengycin only by using bacillus wild type (also producing other lipopeptides). is this possible to get only fengycin? if yes! then which protocol is available and best suited for...

04 May 2018 9,136 0 View

How to study expression of lipopeptide genes in Biocontrol bacteria?

how can i check the expression of lipopeptide genes in bio-control bacterial during antagonism with fungal pathogen. need suggestion through literature.

03 April 2018 7,147 3 View

Howto Prepare samples for scanning electron microscope?

i wanted to analyse the effect of biocontrol bacteria on fungal hypea during antagonism by using Scanning electron microscope (SEM) but i am facing a problem of collecting the fungal hyphea form...

03 April 2018 5,389 7 View

How can i purify polypeptides from the crude extracts using HPLC?

i have extracted some bacterial peptides and i wanted to purify them using HPLC but i dont know how can i purify these peptides. i need suggestions for doing this work. what program should i use...

03 April 2018 2,092 5 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Giorgia Pertile

MagMAX™-96 Total RNA Isolation Kit (ThermoFischer, USA)

Ayaz Farzand

@ Giorgia Pertile is this possible to isolate RNA from the fungal mycelia growing on the petri plate by just scraping it ?

Yes. You collect the colony from the Petri dish by the loop. But you must be careful to take only the mycelium and not the medium.

@ Giorgia Pertile thank you so much for your time and suggestion.

Your read the method in our attached paper.