i am trying to detect bacilysin by MALDI-TOF/MS in the cultural filtrates of bacillus strains but i am unable to detect it. is there any reason for not detecting bacilysin in crude cultural filtrates of bacillus.
Hello, Bacilysin is non-ribosomally synthesised dipeptide that consists of L-alanyl and anticapsin units linked by a peptide bond.
May be the component of bacilysin missing in the MS analysis was alanine. Analysis involving bacilysin, the lowest m/z by the mass spectrometer may be not low enough to detect alanine. If you have base peak m/z 182.1 instead of m/z 271.1 (the bacilysin peak), would represent a loss of one alanine (89 Da).
May be this is the reason.
Karen A. Darbinyan Thanks alot for you answer, please provide me the reference if you have about this one. the lowest mass i am detecting through MALDI-TOF is 803 and bacilysin has 271 mass. kindly provide me a paper which states the point you have written above.
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