I have a problem with restriction digestion ?

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Why the expression of control in qRT-PCR analysis always equal to 1?

i have been doing qPCR analysis for some Pathogenicity related genes in Fungus but when i start calculating the fold changes using given protocol. the expression of control become 1 always. can...

05 June 2019 1,290 9 View

How to predict plant growth promoting genes in the genome of Bacillus strains?

i wanted to study the genomic basis of Plant growth promotion in the genome of bacteria. Is there any software or website which can predict the genes involved directly or indirectly in promotion...

03 April 2019 8,989 4 View

Can hydrocholoric acid be used as a replacement of Picric acid?

I wanted to test the ability of bacteria to produce Hydrogen cyanide. In the procedure, different scientist have used Picric acid but we can't use this acid in our lab so i want to know if HCL can...

03 April 2019 6,415 1 View

Is it possible to detect Bacilysin by MALDI-TOF/MS in culture filtrates of Bacillus strains?

i am trying to detect bacilysin by MALDI-TOF/MS in the cultural filtrates of bacillus strains but i am unable to detect it. is there any reason for not detecting bacilysin in crude cultural...

31 December 2018 1,728 3 View

Do lipopeptides effect pathogenecity genes of a fungus during direct antagonism?

i have been working on mechanism by which bacillus strains can fight with fungal pathogens when they come in direct contact to each other. it has been well documented that antibiosis plays an...

31 December 2018 2,002 3 View

If there any specific protocol for extracting fengycin only ?

i wanted to get Fengycin only by using bacillus wild type (also producing other lipopeptides). is this possible to get only fengycin? if yes! then which protocol is available and best suited for...

04 May 2018 9,136 0 View

How to get fungal colony from solid media for extraction of RNA?

i wanted to study the expression of pathogenicity related genes of fungal pathogen during antagonism. for this i have to isolate RNA from the fungal colony being cultured with antagonist but i am...

04 May 2018 812 5 View

How to study expression of lipopeptide genes in Biocontrol bacteria?

how can i check the expression of lipopeptide genes in bio-control bacterial during antagonism with fungal pathogen. need suggestion through literature.

03 April 2018 7,147 3 View

Howto Prepare samples for scanning electron microscope?

i wanted to analyse the effect of biocontrol bacteria on fungal hypea during antagonism by using Scanning electron microscope (SEM) but i am facing a problem of collecting the fungal hyphea form...

03 April 2018 5,389 7 View

How can i purify polypeptides from the crude extracts using HPLC?

i have extracted some bacterial peptides and i wanted to purify them using HPLC but i dont know how can i purify these peptides. i need suggestions for doing this work. what program should i use...

03 April 2018 2,092 5 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Is there any expert in Smart PLS who can help me?

Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?

01 March 2021 8,212 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View