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Questions related from Ayaz Farzand
i have been doing qPCR analysis for some Pathogenicity related genes in Fungus but when i start calculating the fold changes using given protocol. the expression of control become 1 always. can...
06 June 2019 1,399 9 View
i wanted to study the genomic basis of Plant growth promotion in the genome of bacteria. Is there any software or website which can predict the genes involved directly or indirectly in promotion...
04 April 2019 9,060 4 View
I wanted to test the ability of bacteria to produce Hydrogen cyanide. In the procedure, different scientist have used Picric acid but we can't use this acid in our lab so i want to know if HCL can...
04 April 2019 6,510 1 View
i am trying to detect bacilysin by MALDI-TOF/MS in the cultural filtrates of bacillus strains but i am unable to detect it. is there any reason for not detecting bacilysin in crude cultural...
01 January 2019 1,800 3 View
i have been working on mechanism by which bacillus strains can fight with fungal pathogens when they come in direct contact to each other. it has been well documented that antibiosis plays an...
01 January 2019 2,106 3 View
i have been doing restriction digestion but after digestion when i run the gel, the band is there but not smooth as in control. what can be the problem with this kind or band?
07 July 2018 2,509 2 View
i wanted to get Fengycin only by using bacillus wild type (also producing other lipopeptides). is this possible to get only fengycin? if yes! then which protocol is available and best suited for...
05 May 2018 9,208 0 View
i wanted to study the expression of pathogenicity related genes of fungal pathogen during antagonism. for this i have to isolate RNA from the fungal colony being cultured with antagonist but i am...
05 May 2018 883 5 View
i have extracted some bacterial peptides and i wanted to purify them using HPLC but i dont know how can i purify these peptides. i need suggestions for doing this work. what program should i use...
04 April 2018 2,161 5 View
how can i check the expression of lipopeptide genes in bio-control bacterial during antagonism with fungal pathogen. need suggestion through literature.
04 April 2018 7,228 3 View
i wanted to analyse the effect of biocontrol bacteria on fungal hypea during antagonism by using Scanning electron microscope (SEM) but i am facing a problem of collecting the fungal hyphea form...
04 April 2018 5,462 7 View
