Why the expression of control in qRT-PCR analysis always equal to 1?

i have been doing qPCR analysis for some Pathogenicity related genes in Fungus but when i start calculating the fold changes using given protocol. the expression of control become 1 always. can...

05 June 2019 1,290 9 View

How to predict plant growth promoting genes in the genome of Bacillus strains?

i wanted to study the genomic basis of Plant growth promotion in the genome of bacteria. Is there any software or website which can predict the genes involved directly or indirectly in promotion...

03 April 2019 8,989 4 View

Can hydrocholoric acid be used as a replacement of Picric acid?

I wanted to test the ability of bacteria to produce Hydrogen cyanide. In the procedure, different scientist have used Picric acid but we can't use this acid in our lab so i want to know if HCL can...

03 April 2019 6,415 1 View

Is it possible to detect Bacilysin by MALDI-TOF/MS in culture filtrates of Bacillus strains?

i am trying to detect bacilysin by MALDI-TOF/MS in the cultural filtrates of bacillus strains but i am unable to detect it. is there any reason for not detecting bacilysin in crude cultural...

31 December 2018 1,728 3 View

Do lipopeptides effect pathogenecity genes of a fungus during direct antagonism?

i have been working on mechanism by which bacillus strains can fight with fungal pathogens when they come in direct contact to each other. it has been well documented that antibiosis plays an...

31 December 2018 2,002 3 View

I have a problem with restriction digestion ?

i have been doing restriction digestion but after digestion when i run the gel, the band is there but not smooth as in control. what can be the problem with this kind or band?

06 July 2018 2,438 2 View

How to get fungal colony from solid media for extraction of RNA?

i wanted to study the expression of pathogenicity related genes of fungal pathogen during antagonism. for this i have to isolate RNA from the fungal colony being cultured with antagonist but i am...

04 May 2018 812 5 View

How to study expression of lipopeptide genes in Biocontrol bacteria?

how can i check the expression of lipopeptide genes in bio-control bacterial during antagonism with fungal pathogen. need suggestion through literature.

03 April 2018 7,147 3 View

Howto Prepare samples for scanning electron microscope?

i wanted to analyse the effect of biocontrol bacteria on fungal hypea during antagonism by using Scanning electron microscope (SEM) but i am facing a problem of collecting the fungal hyphea form...

03 April 2018 5,389 7 View

How can i purify polypeptides from the crude extracts using HPLC?

i have extracted some bacterial peptides and i wanted to purify them using HPLC but i dont know how can i purify these peptides. i need suggestions for doing this work. what program should i use...

03 April 2018 2,092 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View