For doing an expression study, first you need to decide the gene of your interest and search/develop qRT-PCR primers for that particular gene. After doing this, you need to design an experiment in which temples will be collected with and without bacterial-fungal interactions during certain time points according to your experimental design. By selecting any house keeping genes, you could perform the expression analysis of with/without interaction samples. The quantification of your data would point you towards the expression profile for the gene analyzed.

For doing the expression of lipopeptides genes, first see the type of lipopeptide. it may be surfactin, iturin and fengycin or a novel lipopeptide. sequence/identify the corresponding amino acids present in the lipopeptide. after that design primer accordingly and make a clone. Further express it in a appropriate host system.selection of primer is very important because each lipopeptide have its own subtypes with minor changes. there are lot of papers available so search on google for further help.

Dr. Khem

Dear

We can follow this link which handle bio-control mechanism for a genus like Bacillus..

Kind regard