How to do enzyme activity test?

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Nick Chacos

There are a lot of nonspecific fluorogenic protease substrates. We conjugated FITC to BSA and really saturated it which quenched the fluorescence. When hydrolyzed, there was a dramatic increase in signal.

Just looking around, ANASPEC has a substrate used for trypsin, cathepsin, etc. AS-60323. If you think it doesn't work with papain, look around. There are lots of similar offerings.

W.J. Colonna

Nov. 21, 2024

Dear Wahyu,

Attached is a procedure from Sigma-Aldrich that has a method for assaying papain.

I hope this information helps you.

Good luck w/ your research.

William J. Colonna Dept. Food Science & Human Nutrition, Iowa State University, Ames, Iowa, USA [email protected]

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Papain Assay Procedure.pdf

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