Is an encrypted dataset with Microsoft SEAL compatible with Cloudsim simulator??

Hello, I would like to do homomorphic encryption using Microsoft SEAL on my dataset and then use it as an input to the Cloudsim simulator to do clustering on. Is it possible and compatible with...

16 February 2021 3,915 1 View

What is the best Information Managment Software for higher education student ex Master student?

I need to build a tracking system for Post Graduate student. The aimed software helps admistative team to record deadlines, generate documents depending on pre input data in registeration phase...

26 December 2020 5,396 3 View

Ellagic acid dissolving?

Can anyone help me with dissolving ellagic acid? Its solubility is about 0.8 mg/ml, I try half this amount but it does not solve

23 December 2020 9,532 3 View

Can I see nanoparticles using optical microscope with stage micrometer?

I am working on Solid Lipid Nanoparticles I am doing MPhil research I am preparing a formulation My topic is Preparation AND Evaluation of Indomethacin Loaded Solid Lipid Nanoparticles for...

27 October 2020 9,496 4 View

Can you recommend a textbook for DSC technique?

Dear colleagues, can you please recommend me a good literature specific on the thermal analysis by DSC technique from the basic principles to advanced applications and interpretation of curves...

08 May 2020 2,988 10 View

Are children will be psychologicaly affected more than adults by the pandemic of Covid-19?

Fear and anxiety disorders are come in children and are directly affected affected by the environmental condition (parents reaction, peers, media).

05 April 2020 8,202 33 View

How to dilute an oil without changing its effect?

I want to have different concentrations of volatile oils, which of course do not mix with water. how it would be?

18 January 2020 6,502 4 View

How to mesh high pressure gradient regions?

I am simulating injector nozzel to see cavitations and want to validate Winklhofer results using Mohan's paper(attached in files). I simulated flow for simple geometry (attached in files) with...

15 January 2020 3,429 1 View

Please does anyone know how to add a tertiary Y-AXIS on a graph?

I am trying to plot keratinase activity, protein concentration and pH changes against incubation time in one graph but then does anyone know how to add three Y-AXIS in one graph? what I know of...

18 October 2019 7,876 3 View

What happens if negative SiV center in diamond is excited with a nonresonant laser light, say 514nm?

Exciting negative SiV center with 737 nm laser pulse, resonant with the D1 transition is generally done for measuring fluorescence. What if laser light of shorter wavelength is used , say 514 nm?

08 August 2019 7,314 1 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

How to do the calculation for super oxide dimutase assay and how to calculate the R squared value in regression equation for catalase assay?

i have the enzymatic antioxidant assay by following the method of Misra and Fridovich, 1972 for super oxide dimutase and Luck, 1974 for catalase assay can any help me how to the calculation for...

11 February 2021 2,241 3 View

CAT assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional catalase enzymatic assay in a 96 well microplate, for cell line and/or C.elegans spectrophotometric measurements. Thanks!

11 February 2021 7,273 3 View

No title

Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

What are some common oxidants used to oxidize iron sulfur clusters in proteins for techniques like EPR?

I have used potassium ferricyanide (which is likely the most common) extensively in the past, but I am curious to know if there are other oxidants that work well. I need to generate the oxidized...

29 January 2021 9,803 4 View

Papain Enzyme Assay...?

Hi all, I would like to know how can I estimate the activity of the enzyme, papain, which is used in reactions as crude latex extract of papaya. Please share some procedures if anybody having...

26 January 2021 569 4 View