Soner is exactly right - knocking down the protein with siRNA is a standard procedure (see examples at the bottom here: https://altogen.com/) for looking at the effects of protein inhibition. Adding protein-blocking aptamers is another method, but siRNA-based silencing is a well-researched technique that has been crucial to many studies.
Soner is exactly right - knocking down the protein with siRNA is a standard procedure (see examples at the bottom here: https://altogen.com/) for looking at the effects of protein inhibition. Adding protein-blocking aptamers is another method, but siRNA-based silencing is a well-researched technique that has been crucial to many studies.
If your protein has special function, you can use indirect assay for evaluation of inhibitor efficacy. For example, if your protein is a trans-membrane enzyme , Km and Vmax if enzyme after and before of inhibitor challenge maybe good index for your purpose.