Conservación de esporas de hongos micorricicos arbusculares?

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How Can I clain a removal of a co-author?

I would like to make a correction in a co-author in your paper which is wrong. This name is also in other papers of Kenton Ko. Zey Ko is not a Co-author . The correct name is Zdenka...

24 February 2021 3,476 4 View

Would anyone have a sample survey questionnaire for an AHP-TOPSIS study?

I am currently working on my undergraduate thesis project and we are trying to compare the AHP method with the AHP-TOPSIS method. I have already found the format for an AHP survey questionnaire,...

03 January 2021 7,213 3 View

Will -80 freezer cause damage to enzymes?

The typical guidance on storing enzymes is to keep them in a -20C freezer, but would storing aliquots of enzyme for long-term storage in a -80C freezer cause damage that would result in the enzyme...

03 December 2020 7,550 3 View

Does anyone experienced EDTA salts precipitation in DMSO?

I added 2 mL of 0.1 M EDTA aqueous solution pH 6, to a solution of 3 mL pure DMSO and instantaneously I observed salt precipitation in solution. Is this happening because the pH of the EDTA...

17 October 2020 6,329 1 View

Did anyone try to measure the thickness of films with fluorescent peptides immobilized by ellipsometry?

I immobilize fluorescent peptide on chitosan films and when I try to measure the thickness of the film with ellipsometry, the software says that I have a film with 2 nm or less. However, with...

01 October 2020 3,702 3 View

Is sytematic review methodology adoped in demographic and population studies?

can a demographer employed the systematic review methodology in their research writing?

19 September 2020 5,242 4 View

How to run Targeted MD simulation using NAMD/ GROMACS?

I want to do targeted MD simulation on a protein (400 amino acids). Can anyone suggest me any good tutorial? Thanks in advance.

17 September 2020 342 2 View

Does anyone sell/know how to make 5 M sodium acetate solution?

I am trying out a new type of binding buffer for DNA extraction and the protocol calls for 5 M sodium acetate, but I cannot find anywhere that sells a stock solution that highly concentrated. The...

13 August 2020 5,149 5 View

Impact of digitalization on Mechanical Engineering?

I am currently researching the impacts of components of digital transformation and Industry 4.0 (optimization, automation, digitalization) on the fairly old field of mechanical engineering. Has...

27 June 2020 2,350 13 View

Impact of data-driven industry and digitalization on Mechanical Engineering field?

I am currently researching the impacts of components of digital transformation and Industry 4.0 (optimization, automation, digitalization) on the fairly old field of mechanical engineering. Has...

24 June 2020 2,360 2 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

Extraction of DNA from insect samples conserved in silica gel?

Hi everybody, can anybody suggest how to extract DNA from insect samples conserved in silica gel (in my case, mosquitoes)? Does anybody know a commericial kit which does that or a particular...

10 February 2021 3,392 4 View

Any specific reason, how sclerotium rolfsii can grow out of Petridish?

Is it related to nutrition or any other reason? Any other fungus have similar kind of growth pattern?

09 February 2021 4,357 4 View

MAD or boxplot to detect outliers in non normally distributed data for latency and distance in animal cognition?

I am studying animal cognition and I have data about animals' latency to approach a cue and animals' distance from the cue in a test. Data are not normally distributed and when I do scatterplots...

04 February 2021 503 3 View