In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

Check for binding of crRNA to tracrRNA?

For my internship I have to develop crispr-cas9 guides for certain genes. One of the things I have to validate in silico, is whether or not the crRNA has the ability to bind the tracRNA sequence...

08 September 2019 1,631 1 View

How efficient in Knock-in genome editing in murine HSPCs?

Based a few hours search, there is to date no established protocol for transfecting murine HSPCs for generating knock-ins of several kb size with reported efficiencies. All I can find is...

05 June 2019 3,436 1 View

How many bands do I expect to observe after agarose gel electrophoresis of crRNA:tracrRNA duplex?

Hi all! Does anyone tried to perform agarose gel electrophoresis of crRNA:tracrRNA duplex after annealing? How many bands do I expect to observe if the annealing is successful and how many if the...

01 February 2019 3,937 2 View

Can I detect any amplicon less than 50bps with qPCR?

I want to count the amount of crRNA expressed in a plasmid with Ecoli. I am not sure whether I can use a RT-qPCR to do it. The length is between 50 - 100bps.

08 September 2018 1,113 6 View

Do you need to add tracrRNA to a plasmid with cas9 if you include gRNA?

Okay, I haven't used crispr/cas9 in a couple years so I'm starting to get confused as I look over my old notes and the new literature (and the plasmids on AddGene!). I thought gRNA was a synthetic...

08 September 2018 5,541 1 View

What is the difference between tracrRNA and crRNA?

28 March 2018 2,829 3 View

Can 2 plasmids for crispr be of the same kind,please help??

The cas 9 and sgrna will go into a plant plasmid The donor template will be also go in plant plasmid also- pcambia 1201, Both are going to fix a defective gene in chlorella-microalgae plant...

02 March 2018 3,928 19 View

What is the difference between tracrRNA and crRNA?

02 March 2018 7,733 6 View

Why have I a presence of a miRNA in Jurkat cells that I have specifically mutated, insering a donor DNA?

Hello, I have a strange problem. In my lab we are trying to use CRISPR/Cas9 technique to create a Knock-out of a miRNA in Jurkat cells ( T cells) . In detail I used a crRNA that target...

01 February 2018 5,286 0 View

Looking for a negative control for CRISPR in zebrafish?

I am injecting zebrafish with gRNA targeted to my gene of interest, a plasmid with that codes for a fluorescent protein, and gRNA targeted to the plasmid so that the plasmid inserts into my gene...

07 August 2017 4,895 1 View