How to design crRNA for Cas12a based detection?

02 August 2021 1 5K Report

Can anyone please guide me through how to design crRNA for Cas12a based detectioin.

For IVT purpose where should be the T7 promoter and where I should add repeats.

https://www.neb.com/faqs/2018/05/03/how-do-i-design-a-guide-rna-for-use-with-engen-lba-cas12a#:~:text=The%20guide%20RNA%20%28crRNA%29%20for%20EnGen%20Lba%20Cas12a,underlined%29%2C%20which%20will%20vary%20depending%20on%20the%20target.

please refer to this website to see the detail of crRNA design.

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