Pooja Bhardwaj

5 Questions 7 Answers 0 Followers

Questions related from Pooja Bhardwaj

What is the role of activator in CRISPR/Cas12 based detection?

I have read many research articles where an activator [a stretch of nucleotides (

08 May 2023 4,333 0 View

Why PCR amplification failed with Taq polymerase while giving good result with Pfu polymerase?

I want to amplify a cDNA (1600bp) using Taq polymerase which I have amplified using Pfu polymerase. I have tried all changes I can do like temperature variation, increased MgSO4 concentration...

21 October 2022 9,016 3 View

How to design crRNA for Cas12a based detection?

Can anyone please guide me through how to design crRNA for Cas12a based detectioin. For IVT purpose where should be the T7 promoter and where I should add repeats.

02 August 2021 5,166 1 View

Could anyone please tell me how to prepare cell free extract for enzyme for gram positive bacteria skipping sonication step?

12 May 2016 9,684 3 View

Can anybody tell me how the Inc plasmids are categorized? on what basis inc plasmids were grouped?

Can Anybody tell me how the Inc plasmids are categorized? on what basis inc plasmids were grouped?How many groups and subgroups of Inc plasmids are there?

16 August 2015 7,728 4 View