Based a few hours search, there is to date no established protocol for transfecting murine HSPCs for generating knock-ins of several kb size with reported efficiencies. All I can find is gene-disruption.

Given that human HSC editing has progressed so far, does anybody have any insight if things have been looking up for mice HSCs as well?

I'm interested in extracting HSPCs from a mouse, then knocking in a 2kb gene into the cells and adoptively transferring them back into the mouse. Is that at all possible? I have Cas9 mice available so I won't require any Cas9 protein/plasmid/mRNA, just the crRNA;tracrRNA complex and my template.

Thank you for any input