I tried to generate a knock-out of CYFIP1 in HEK293T using RNP, but was unsucessful. I tried to complex 30nM final conc of sgRNA ( crRNA and trRNA) and 15 nM final conc of Cas9GFP from Sigma. We used TransIT to transfect the cells 10^6 cells and the success of knock-out was checked using T7E1 assay. Does anyone have experience of having generated a knock-out using RNP?