Does anyone know why my CRISPR-Cas12a trans-cleavege efficiency is so low? I refer to someone else's literature and there is almost no difference, but the efficiency of reverse cutting is very low.In the referenced paper，we have used the same ssDNA (the target)，the same crRNA，but the reporter is different.I don't think that this is the key point.We have controled the final molar ratio of Cas12a:crRNA:targrt to 10:10:1. We also used the DEPC water in the each step. To be honest,this is the first time we do the ralative research. I think there maybe something I can't consider well. I hope to receive your help