After generating crRNA+tracrRNA heating at 95C, can it be maintained at -20C and be used in future experiments? Or crRNA+tracrRNA needs to be fresh?

25 November 2021 0 2K Report

I only have used sgRNAs. For the first time I used crRNA+tracrRNA and I kept what was left in the -20C. Can I use it again or is it better to prepare fresh crRNA+tracrRNA?

Thank you :)

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