I would like to nucleofect human BJ1 hTERT immortalised fibroblasts with a RNP complex made of Cas9 and a single crRNA+Tracer RNA, using the Amaxa 4D cell nucleofector device. Is there a standard program to do such experiment? On the web, there's a paper that described the use of program DT-130, but that paper used Cas9 and sgRNA from two plasmids, not directly RNPs. Any help will be appreciated!