How to do the synthesis of crRNA for Cfp1/CRISPR?

26 April 2019 0 1K Report

Dear All,

I'm looking for the picture of the template structure (example of oligonucleotides) for crRNA synthesis by transcription in vitro by use of HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB).

NEB is providing only the description of sgRNA synthesis : https://www.neb.com/protocols/2015/11/24/sgrna-synthesis-using-the-hiscribe-quick-t7-high-yield-rna-synthesis-kit-neb-e2050

I'm looking for the similar one for Cfp1 (Cas12).

Regards,

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