Hello everyone!!
I am trying to grow MIN6 cells, but I am unable to. The growth rate is too slow. I can hardly see 5-6 cells in the plate. Also the morphology of the cells is highly irregular. I am using DMEM plus 12 percent FBS with high glucose. Any suggestions are highly welcomed.
TIA
Thank you for the suggestion. Is this specifically for min6 cells? What is the trypsinization procedure you use?
Hello
You can culture MIN6 cells in DMEM-High Glucose medium, 15% FBS, 1% P/S, 1% Glutamine (2mM), 20 mM HEPES (diluted from 1M HEPES purchased from Sigma) and 50-55 μM beta-mercaptoethanol (1.75-2.0 μl/ 500 ml of medium). Change medium every 2-3 days. Moreover, MIN6 cells are very sensitive to FBS, therefore, it is important to check a few batches of FBS and see whether the cells grow better with a certain batch of FBS.
Good luck
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