How to culture MIN6 cells?

Hello everyone!! I am trying to grow MIN6 cells, but I am unable to. The growth rate is too slow. I can hardly see 5-6 cells in the plate. Also the morphology of the cells is highly irregular. I...

07 August 2018 7,230 3 View

How to write a thesis title?

Can a thesis title be a question?

08 September 2017 7,041 2 View

Do Min6 cell lines adhere to glass slides?

I wish to grow Min6 cell lines on glass slides for imaging experiments. Are these cells adherent or do I need to coat the slides before seeding cells on to them?please advise

01 February 2017 5,716 3 View

Can anyone suggest me a protocol for analysis of ROS in aqeous solutions?

Hi I want to measure ROS in a aqueous solution containing a protein and ROS generating agents in buffered solutions. Is there a protocol for the same/ and is it ok to use DCFH-DA for this? Please...

31 December 2016 1,401 0 View

What is the optimal staining procedure for SDS-PAGE?

I have a protein to be expressed with an apparent MW of 18.4 Kda.For that I used 13% resolving gel.I use an unstained protein ladder.After the run was over, I stained the gel with commasie.The...

06 July 2016 7,997 5 View

How long a stock solution of amyloid proteins is stable in DMSO?

I have stored a stock solution of an amyloid protein in DMSO for a long time. How long it will be stable without aggregating or forming amyloids, so that I can use it for further studies/experiments.

05 June 2016 7,469 5 View

What should be the optimum protein load (volume and concentration) when purifying a protein by sephadex G 100 , Column chromatography?

31 December 2015 7,307 3 View

How would I do MM-PBSA analysis after MD simulations in GROMACS?

31 December 2015 6,869 3 View

What is microfluidic protein sizing?

Can anyone explain me what is the meaning and principle of microfluidic protein sizing

11 December 2015 4,521 4 View

How might I calculate Guanidinium concentration in molecular dynamics simulation of proteins?

Hi everyone!! While performing the MD simulations on proteins and studying their unfolding by Guanidium, How should one determine the guanidium concentration with regard to the number of molecules...

06 July 2015 4,373 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View