How to culture MIN6 cells?

Hello everyone!! I am trying to grow MIN6 cells, but I am unable to. The growth rate is too slow. I can hardly see 5-6 cells in the plate. Also the morphology of the cells is highly irregular. I...

07 August 2018 7,230 3 View

Do Min6 cell lines adhere to glass slides?

I wish to grow Min6 cell lines on glass slides for imaging experiments. Are these cells adherent or do I need to coat the slides before seeding cells on to them?please advise

01 February 2017 5,716 3 View

Can anyone suggest me a protocol for analysis of ROS in aqeous solutions?

Hi I want to measure ROS in a aqueous solution containing a protein and ROS generating agents in buffered solutions. Is there a protocol for the same/ and is it ok to use DCFH-DA for this? Please...

31 December 2016 1,401 0 View

What is the optimal staining procedure for SDS-PAGE?

I have a protein to be expressed with an apparent MW of 18.4 Kda.For that I used 13% resolving gel.I use an unstained protein ladder.After the run was over, I stained the gel with commasie.The...

06 July 2016 7,997 5 View

What are the criteria while using a protease inhibitor?

06 July 2016 5,072 5 View

How long a stock solution of amyloid proteins is stable in DMSO?

I have stored a stock solution of an amyloid protein in DMSO for a long time. How long it will be stable without aggregating or forming amyloids, so that I can use it for further studies/experiments.

05 June 2016 7,469 5 View

What should be the optimum protein load (volume and concentration) when purifying a protein by sephadex G 100 , Column chromatography?

31 December 2015 7,307 3 View

How would I do MM-PBSA analysis after MD simulations in GROMACS?

31 December 2015 6,869 3 View

What is microfluidic protein sizing?

Can anyone explain me what is the meaning and principle of microfluidic protein sizing

11 December 2015 4,521 4 View

How might I calculate Guanidinium concentration in molecular dynamics simulation of proteins?

Hi everyone!! While performing the MD simulations on proteins and studying their unfolding by Guanidium, How should one determine the guanidium concentration with regard to the number of molecules...

06 July 2015 4,373 3 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View