Can anyone explain me what is the meaning and principle of microfluidic protein sizing
Hello
Look at the papers here , will help you
Good Luck
http://faculty.washington.edu/yagerp/microfluidicstutorial/basicconcepts/basicconcepts.htm#
http://link.springer.com/chapter/10.1007/1-4020-3103-3_78#close
http://www.nsti.org/publications/Nanotech/2003/pdf/M2104.pdf
https://books.google.com.sa/books?id=MC5NVC7YSCcC&pg=PA393&lpg=PA393&dq=microfluidic+protein+sizing&source=bl&ots=DH1JDoluob&sig=YZMvrPgxYO2n0PVOPWZHGwpXyKw&hl=ar&sa=X&ved=0ahUKEwiapa2gqL3JAhUITBQKHX-QCeM4ChDoAQgbMAA#v=onepage&q=microfluidic%20protein%20sizing&f=false
Dear Shreyasi,
In addition to the relevant publications you have received from Saleh, please attached please find a comprehensive review which discusses the origins and the future of microfluidics. I have copied the review abstract for quick view.
The origins and the future of microfluidics
George M. Whitesides1
The manipulation of fluids in channels with dimensions of tens of micrometres — microfluidics — has emerged as a distinct new field. Microfluidics has the potential to influence subject areas from chemical synthesis and biological analysis to optics and information technology. But the field is still at an early stage of development.
Even as the basic science and technological demonstrations develop, other problems must be addressed: choosing and focusing on initial applications, and developing strategies to complete the cycle of development, including commercialization. The solutions to these problems will require imagination and ingenuity.
Hoping this will be helpful,
Rafik
Here are some interesting papers : https://scholar.google.com/scholar?hl=fr&q=microfluidic+protein+sizing&btnG=&lr=
Microfluidic diffusional sizing (MDS) exploits the propensity of fluids to flow in a laminar state when confined to small (micrometre) length scales. Consequently, when two streams of fluid, one containing particles of interest and the other containing exclusively buffer, meet in a microfluidic channel, there is no convective mixing. Transport of the molecules into the buffer perpendicular to the direction of the flow lines proceeds, in the absence of an applied force, exclusively via diffusion (Weigl, Science, 1999). The rate of diffusion is related to the hydrodynamic radius (Rh) of the particles, and so provided that the streams can then be separated, and the quantity of particles in each stream subsequently quantified, the average Rh of the particles in solution can be calculated.
In the case of protein, a detection chemistry must then be introduced to quantify the protein in each stream. In her 2015 paper, Yates showcases the combination of MDS with a latent amine reactive label. https://www.nature.com/articles/nchem.2344
You can see a schematic drawing of how this works here: https://www.fluidicanalytics.com/resources/faq/what-microfluidic-diffusional-sizing/
This approach has since been refined and incorporated into the Fluidity One instrument from Fluidic Analytics
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