17 Questions 23 Answers 0 Followers
Questions related from Shreyasi Asthana
Hello everyone!! I am trying to grow MIN6 cells, but I am unable to. The growth rate is too slow. I can hardly see 5-6 cells in the plate. Also the morphology of the cells is highly irregular. I...
08 August 2018 7,364 3 View
Can a thesis title be a question?
09 September 2017 7,110 2 View
I wish to grow Min6 cell lines on glass slides for imaging experiments. Are these cells adherent or do I need to coat the slides before seeding cells on to them?please advise
02 February 2017 5,774 3 View
Hi I want to measure ROS in a aqueous solution containing a protein and ROS generating agents in buffered solutions. Is there a protocol for the same/ and is it ok to use DCFH-DA for this? Please...
01 January 2017 1,477 0 View
How should I choose an effective protease inhibitor which can can be used for the purification of a range of proteins to be purified by Q-sepharose or Ni-NTA or SP-sepharose?
07 July 2016 5,150 5 View
I have a protein to be expressed with an apparent MW of 18.4 Kda.For that I used 13% resolving gel.I use an unstained protein ladder.After the run was over, I stained the gel with commasie.The...
07 July 2016 8,077 5 View
I have stored a stock solution of an amyloid protein in DMSO for a long time. How long it will be stable without aggregating or forming amyloids, so that I can use it for further studies/experiments.
06 June 2016 7,538 5 View
how would I do MM-PBSA analysis after MD simulations in GROMACS Is there a tool or software for analysing the MM-PBSA of protein ligand complexes
01 January 2016 6,966 3 View
What should be the optimum protein load (volume and concentration) when purifying a protein by sephadex G 100 , Column chromatography Any suggestions would be highly appreciated :)
01 January 2016 7,388 3 View
Can anyone explain me what is the meaning and principle of microfluidic protein sizing
12 December 2015 4,633 4 View
Hi everyone!! While performing the MD simulations on proteins and studying their unfolding by Guanidium, How should one determine the guanidium concentration with regard to the number of molecules...
07 July 2015 4,488 3 View
Hi everyone.. With my previous discussion I would like to add this point I'm trying to express and purify a 6X his tagged protein, I am getting a band on SDS PAGE at the desired mol wt size but I...
01 January 2015 5,337 10 View
Hi all I am trying to isolate a seed protein. I did ammonium sulphate precipitation followed by dialysis against MilliQ to remove the salts. After dialysis, the protein is getting too...
09 September 2014 3,930 7 View
Hi I want to run an SDS-PAGE of nanoparticles synthesized from plant extracts,to see the(if at all) proteins are getting adsorbed onto the nanoparticles.I have done this experiment twice but I do...
07 July 2014 5,739 5 View
I have to sub-clone a gene from one vector into another. However I cannot find the correct plasmid with correct MCS. What do I do?
04 April 2014 661 2 View
Could someone please explain me the difference between BL21DE3 and BL21DE3 pLysS and lacUV5 promoters and how they function?
02 February 2014 4,893 1 View
We always use phenol chloroform for plasmid DNA isolation. But how does phenol denature proteins and what does choloroform do?
01 January 2014 9,042 3 View
