How to convert a .fasta file to fastq file ?

I have a FASTA file, how can i convert it to a FASTQ file via command line or by any online converter. The existing online converter is giving error and is unable to parse my fasta file.

11 December 2018 9,878 15 View

How to map Illumina probe set ID to Gene ID?

Is there any computational tool/software/program code to convert/map illumina probe set IDs to gene IDs for a large data file...

09 October 2017 9,777 1 View

How to extract or unzip HT_HG-U133A files for further anlysis of gene expression data?

07 August 2017 995 3 View

What is the best approach for detecting functional modules in gene expression data ?

What are some of the best approaches to find out functional modules in gene expression data .

04 May 2017 7,960 4 View

How DNA Circuits be used to stop cancer progrssion?

Can DNA Circuits be used to make nano auto triggered bombs to stop cancerous cells from growing when they come in contact with them.

08 September 2016 2,549 1 View

How to represent cancer in differential equation model ?

06 July 2016 4,655 2 View

How can Q3 accuracy of secondary structure of proteins be calculated ?

How is the Q3 accuracy or three state accuracy of secondary structure of proteins calculated. In some texts it is written that it the no. of correctly predicted secondary conformation of amino...

02 March 2016 1,055 5 View

Is Python better than Perl ?

08 September 2015 8,725 2 View

Does drinking excess coffee prevent sleep paralysis ?

08 September 2015 5,771 4 View

How much voltage is generated in brain when synapse occurs ? Is it sufficient to light up a bulb ?

How does electrons flow from one synaptical area to another during synapse ?

08 September 2015 3,750 1 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View