How to convert a .fasta file to fastq file ?

I have a FASTA file, how can i convert it to a FASTQ file via command line or by any online converter. The existing online converter is giving error and is unable to parse my fasta file.

11 December 2018 9,878 15 View

How to construct a 5-node PPI-TF-mirna-gene regulatory network?

I have some genes known to be involved in Oral Submucous Fibrosis (OSF) progression and also their PPI network along with gene-gene interaction. I want to construct a 5-node Feed forward loop with...

09 October 2018 1,786 0 View

How to map Illumina probe set ID to Gene ID?

Is there any computational tool/software/program code to convert/map illumina probe set IDs to gene IDs for a large data file...

09 October 2017 9,777 1 View

How to extract or unzip HT_HG-U133A files for further anlysis of gene expression data?

07 August 2017 995 3 View

What is the best approach for detecting functional modules in gene expression data ?

What are some of the best approaches to find out functional modules in gene expression data .

04 May 2017 7,960 4 View

How DNA Circuits be used to stop cancer progrssion?

Can DNA Circuits be used to make nano auto triggered bombs to stop cancerous cells from growing when they come in contact with them.

08 September 2016 2,549 1 View

How can Q3 accuracy of secondary structure of proteins be calculated ?

How is the Q3 accuracy or three state accuracy of secondary structure of proteins calculated. In some texts it is written that it the no. of correctly predicted secondary conformation of amino...

02 March 2016 1,055 5 View

Is Python better than Perl ?

08 September 2015 8,725 2 View

Does drinking excess coffee prevent sleep paralysis ?

08 September 2015 5,771 4 View

How much voltage is generated in brain when synapse occurs ? Is it sufficient to light up a bulb ?

How does electrons flow from one synaptical area to another during synapse ?

08 September 2015 3,750 1 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

How to handle studies which report perfect effect sizes in a meta-analysis using Pearson's r?

I'm in the process of updating a meta-analysis and in one of the new studies, effect sizes are reported as Tau-U. Two of the effect sizes are reported as Tau-U = 1.00. I am using Andy Field's...

24 February 2021 2,265 3 View