I have HT_HG-U133A and Normalized gene RNAseq data, but both in zipped format.When i unzip them they are not opening with any softwares . How should i make useful for further gene expression analysis in R..
Since you have downloaded raw data from the author's work, It must be in Raw format or you could say .SRA format, to convert this to any other format so that machine or R programme can use it, Convert your file from .SRA to fastq or .fa format, which is a standard format to work on Raw data. You can use SRA toolkit to convert the file. Note: The toolkit only works with linux and it requires higher CPU to process it, if you have any server , upload the file onto it and use the toolkit on it. Time may vary, depending on your data. If you have 2.5 GB file , it may take 5 to hours with high power CPU.
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