How to study anticancer property from plant-based sources, without using animal cell culture facility?

I am working on developing the plant-based drug, especially on anticancer product line development, characterization. Instead of chemical synthetic materials, it's too expensive, side affect and...

03 April 2018 8,565 2 View

How can represent in a table or graph in DPPH, Reducing Power H2O2, Total phenol, and superoxide radical scavenging assay with the level of IC50 %?

I am working on antioxidant capacity characterization of wild fruits. I have generated a data by using various methods such as DPPH, Reducing Power Assay and H2O2 assay, Total phenol assay, and...

31 December 2017 6,001 4 View

How to generate doi, I have published research articles, but provide doi individual, Please suggest me how to get it ?

Published research article, but there is individual doi, how to get it, what is procedure for getting to doi. please advise me.

07 August 2017 1,952 6 View

Is there any way to in vitro methods for checking to anticancer properties of plant extracted substance without using animal cell line or MTT assay?

I am working on the phara quantification of plant substance, facing some experimental aspect , alike no availability of animal cell culture setup. So, anticancer assay aspect completely non in...

31 December 2016 3,943 6 View

How to analyse wild collected mushroom, during a field survey , best fit models for statistically proven ?

Field survey, statistically representation with good correlation of present environment or weather etc.

06 July 2016 7,770 3 View

How to use a mushroom as a drug developing tool ?

Mushroom drug discovery, human valuable substance etc.

06 July 2016 7,129 1 View

How to identify poisonous mushroom from edible ?

How to identify poisonous mushroom from edible, by biochemical testing in quick steps ?

06 July 2016 6,319 1 View

No title

02 March 2016 2,753 3 View

What should be the best possible way to study about antimalarial activity of plant extract?

Spectroscopic assay for quick determination Without required human blood culture medium

01 February 2016 7,067 4 View

How to identify bracket macro fungi? Which type of parameter to follow during the identification and characterization?

Read 5 answers by scientists with 3 recommendations from their colleagues to the question asked by Nagendra Kumar Chandrawanshi on Jan 14, 2016

31 December 2015 4,705 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View