How to calibrate a Microscan MS5608A (Elisa reader) instrument, which is not functional in last one year ?

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How to study anticancer property from plant-based sources, without using animal cell culture facility?

I am working on developing the plant-based drug, especially on anticancer product line development, characterization. Instead of chemical synthetic materials, it's too expensive, side affect and...

03 April 2018 8,714 2 View

How can represent in a table or graph in DPPH, Reducing Power H2O2, Total phenol, and superoxide radical scavenging assay with the level of IC50 %?

I am working on antioxidant capacity characterization of wild fruits. I have generated a data by using various methods such as DPPH, Reducing Power Assay and H2O2 assay, Total phenol assay, and...

31 December 2017 6,191 4 View

How to generate doi, I have published research articles, but provide doi individual, Please suggest me how to get it ?

Published research article, but there is individual doi, how to get it, what is procedure for getting to doi. please advise me.

07 August 2017 2,151 6 View

Is there any way to in vitro methods for checking to anticancer properties of plant extracted substance without using animal cell line or MTT assay?

I am working on the phara quantification of plant substance, facing some experimental aspect , alike no availability of animal cell culture setup. So, anticancer assay aspect completely non in...

31 December 2016 4,123 6 View

How to analyse wild collected mushroom, during a field survey , best fit models for statistically proven ?

Field survey, statistically representation with good correlation of present environment or weather etc.

06 July 2016 7,927 3 View

How to use a mushroom as a drug developing tool ?

Mushroom drug discovery, human valuable substance etc.

06 July 2016 7,296 1 View

How to identify poisonous mushroom from edible ?

How to identify poisonous mushroom from edible, by biochemical testing in quick steps ?

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How to assessment of in vitro antiinflammatory activity of plant extract for determining immunologic study ?

In vitro anti-inflammatory perception plant extract immunologic values Quick assessment of bioactive component

02 March 2016 2,953 3 View

What should be the best possible way to study about antimalarial activity of plant extract?

Spectroscopic assay for quick determination Without required human blood culture medium

01 February 2016 7,378 4 View

How to identify bracket macro fungi? Which type of parameter to follow during the identification and characterization?

Read 5 answers by scientists with 3 recommendations from their colleagues to the question asked by Nagendra Kumar Chandrawanshi on Jan 14, 2016

31 December 2015 4,856 5 View

What is the weight of rotavirus A (ng)?

I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?

29 July 2024 3,138 2 View

ELISA sandwich with two monoclonal antibodies ?

hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...

16 July 2024 9,044 10 View

What do I do with dried urine samples?

I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without...

14 July 2024 6,724 0 View

Has anyone used the new E1 Clip tip digital pipettes?

We just purchased a multichannel and a single channel E1 Clip tip pipettes. In our first attempt to use them for an experiment they are not dispensing accurately? The rep told me that...

01 July 2024 1,983 0 View

How do I prepare rat cardiomyocyte cell lysate for ELISA via freeze-thaw method?

Anyone have a protocol for making cell lysate from rat cardiomyocyte pellets to run an ELISA using PBS as the buffer?

23 June 2024 2,264 4 View

What is considered a biological replicate when you are doing an ELISA plate measurement with different cell types?

If cells obtained from different passages, with different plating but harvested on the same day are used in a single plate to take an ELISA measurement (with the given standards and treated...

17 June 2024 6,705 1 View

Different interpolated concentrations?

I used two standards (one came with the ELISA kit and the other I made myself, by preparing a series of known concentrations of the target analyte) on the same plate in my ELISA run. Why are the...

15 June 2024 5,109 1 View

Question about ELISA with the "Cattle IGF 1 (Insulin like growth factor) ELISA Kit" Cat: ELK5630?

Dear fellow researchers, We are conducting an ELISA with the "Cattle IGF 1 (Insulin-like growth factor) ELISA Kit" (Cat: ELK5630). Samples and reagents were prepared following the instruction...

13 June 2024 9,473 0 View

How to convert mIU/mL of beta galactosidase enzyme to pg/ml? I have used ELISA to determine beta gal concentration in mIU/ mL in human serum samples?

We have to report the concentration in pg/ml, the ELISA kit provider did not reveal the specific activity of the enzyme. In that case how to convert the units?

05 June 2024 2,173 5 View

What is Area Under the Curve for detecting SARS-COV-2 neutralizing antibodies?

I have a question about AUC for calculating SARS-CoV-2 neutralizing antibody tests. AUC here is not the Area Under the ROC Curve. After six serial dilutions of human serum, an indirect ELISA was...

05 June 2024 8,191 0 View

Mike Zhang

Contacting the manufacturer's customer support might be helpful.

Nagendra Kumar Chandrawanshi

Thank You, Miike, I have contact manufacturer, but I to want know, from the user for the same instrument.

Khaled Elnagar

If it is not functional in last year...it shall be checked by manufacturer... most of these microscan has auto-adjustment or according to their operating manuals...