I am working on developing the plant-based drug, especially on anticancer product line development, characterization. Instead of chemical synthetic materials, it's too expensive, side affect and having a lot drawback. Presently my laboratory is not efficient for working with animal cell culture assay. So, I am trying to find human similarity model organisms, that can easy to perform with the anticancer assay also overcome with an ethical barrier. Please suggest me .
Aside from the conventional MTT assay which utilizes cell culture components, you can try kinase inhibitor assay such as this one:
https://worldwide.promega.com/resources/pubhub/screening-and-profiling-kinase-inhibitors-with-a-luminescent-adp-detection-platform/
You may also analyze the antioxidant nature of the plant extracts as ROS production has been linked to events occurring in cancer development/progression.
Article DPPH (1,1-Diphenyl-2-Picrylhydrazyl) Radical Scavenging Acti...
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