According to IDT oligo design, Ta Optimum = 0.3 x (Tm of primer) + 0.7 x (Tm of product) – 14.9; where Tm of primer is the melting temperature of the less stable primer-template pair, and Tm of product is the melting temperature of the PCR product. How should I calculate the Tm of my pcr product for the above mentioned formula?
https://sg.idtdna.com/pages/support/faqs/how-do-you-calculate-the-annealing-temperature-for-pcr-#:~:text=The%20optimal%20annealing%20temperature%20(T,the%20melting%20temperature%20of%20the