Carolina Schäfer

3 Questions 3 Answers 0 Followers

Questions related from Carolina Schäfer

Which viability dye could be most suitable for cell sorting?

I need to sort murine NKT hybridomas via TCRb and CD1d double-staining, but I also want to discriminate live from dead cells. I have been using Blue live/dead fixable stain by Invitrogen in flow...

11 September 2016 8,521 6 View

Can anyone help with in vitro CD40 ligation in murine dendritic cells (coculture with J558L-CD40L transfected cell line)?

I'm trying to characterize dexamethasone-modulated MPLA-stimulated murine dendritic cells for a model of arthritis and so far I've obtained a tolerogenic profile according to surface molecules...

03 June 2014 6,046 4 View

Concentration for blocking solution of isotype should be the same for test monoclonal Ab?

I'm carrying murine bone marrow-derived dendritic cells cultures. I was having trouble with the signal of the isotype control for anti-CD11c+ PECy5, because the signal was so intense that when I...

29 May 2013 5,131 1 View