Carolina Schäfer

2 Questions 2 Answers 0 Followers

Questions related from Carolina Schäfer

Which viability dye could be most suitable for cell sorting?

I need to sort murine NKT hybridomas via TCRb and CD1d double-staining, but I also want to discriminate live from dead cells. I have been using Blue live/dead fixable stain by Invitrogen in flow...

11 September 2016 8,458 6 View

Concentration for blocking solution of isotype should be the same for test monoclonal Ab?

I'm carrying murine bone marrow-derived dendritic cells cultures. I was having trouble with the signal of the isotype control for anti-CD11c+ PECy5, because the signal was so intense that when I...

29 May 2013 5,097 1 View