Hrithik Kumar

3 Questions 1 Answers 0 Followers

Questions related from Hrithik Kumar

What is the right gain to be chosen for plotting fluorescence intensity measurement?

I am measuring fluorescence intensity using a Tecan microplate reader from two assay plates with the same samples starting at two different time points. I want to plot both the plate readings in...

23 June 2024 1,042 1 View

Does the annealing temperature changes with different DNA polymerases?

I am doing a PCR, and I checked the annealing temperature of my oligos in the NEB Tm calculator. When I selected Q5 polymerase, it said the annealing temperature was 58 degrees celsius, but when I...

13 June 2022 1,670 3 View

How to calculate Tm of the PCR product?

According to IDT oligo design, Ta Optimum = 0.3 x (Tm of primer) + 0.7 x (Tm of product) – 14.9; where Tm of primer is the melting temperature of the less stable primer-template pair, and Tm of...

13 June 2022 1,820 1 View