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Questions related from Hrithik Kumar
I am doing a PCR, and I checked the annealing temperature of my oligos in the NEB Tm calculator. When I selected Q5 polymerase, it said the annealing temperature was 58 degrees celsius, but when I...
13 June 2022 1,662 3 View
According to IDT oligo design, Ta Optimum = 0.3 x (Tm of primer) + 0.7 x (Tm of product) – 14.9; where Tm of primer is the melting temperature of the less stable primer-template pair, and Tm of...
13 June 2022 1,814 1 View
