Can crude enzyme be used for immobiliazation?

Can crude enzyme be utilized as immobilized enzyme for industrial application if the crude enzyme shows high activity?

13 July 2020 8,672 4 View

From where can I get microglia cell line like N9 or BV-2 in India ( preferably in Kolkata)?

From where can I get microglia cell line like N9 or BV-2 in India ( preferably in Kolkata)?

05 November 2019 7,719 2 View

How to conjugate a dye to the N-terminus of poly-glutamic acid peptide?

Hi! I am trying to conjugate a dye in its NHS ester form to the N-terminus of poly-glutamic acid peptide ( which is already available in the form of a white solid). I tried 3 times and am tring...

27 June 2019 8,798 3 View

Is it necessary to purify PCR products before cycle sequencing?

I will purify the cycle sequencing products using EDTA/EtOH precipitation method before loading to 96 well plate.

30 November 2018 6,932 4 View

In case of Box behnken design reduced quartic model, do I need to mention all the insignificant terms in the ANNOVA table and polynomial equation?

When I try to reduce those terms the R2 value reduces. As a result I have many terms whose sum of squares and df values are 0. The p value and all other values fall in range.

22 May 2018 8,088 4 View

How to measure voltage dependent strain of a thin film without using electric field dependent XRD???

because we have not any facility of electric field dependent XRD but all the papers suggested this electric field dependent XRD. If anyone can explore any idea then please help me

15 April 2018 263 2 View

In an XRD data if the peak are same with ICSD data but intensity is not matched with ICSD data then is anything worng???

My XRD data for NdNiO3 is similar with ICSD data but intensity are not matching with ICSD data

11 March 2018 8,823 4 View

How a core flood experiment for conformance control can be designed?

The limitation we are currently having is that cores of length upto 6 inch can only be used in our system and we can not prepare composite cores.

03 September 2017 9,093 2 View

Can zero field cooled MH loop measured after some times (few months) for same magnetic sample at same temperature be different?

17 February 2017 6,694 5 View

PLD Vs RF sputtering?

How PLD and RF sputtering are different over each other ? Or why one should choose PLD over sputtering except depositing complicated oxides (e.g: super conductors or perovskites). It is well known...

12 October 2016 1,464 4 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

Does increasing antibiotic concentration also increase plasmid production in DH5a E.coli?

Dear all, I am propagating some plasmids with a human gene and an ampicillin resistance gene. My logic is that if ampicillin concentration will be increased, the concentration of plasmid (pSF)...

14 January 2021 779 3 View

Why nitrocefin is preferred over CENTA?

The chromogenic beta-lactamase substrate CENTA is at least five times cheaper than the other famous substrate nitrocefin and can be used with most enzymes, still, nitrocefin is preferred. Any...

17 December 2020 3,735 2 View

Greiner Bio or Corning Costar plates for ELISA?

09 December 2020 237 2 View

How do you confirm your beta receptor antagonist is WORKING before you implement it in a big experiment?

I want to use a beta blocker in vivo but first I want to ensure the drug is working and for how long the effects last after the drug is withdrawn. I'm thinking I can administer the drug and then...

06 December 2020 5,179 1 View

Which should be used for Anxiety : unselective beta blocker or B1 Blocker ?

which would be more effective in treatment or protection from Anxiety : unselective beta blocker as Propranolol or selective beta-1 adrenergic receptor antagonist as Bisoprolol ?

15 November 2020 7,994 2 View

Effect of excess cyclodextrin on drug after cyclodextrin reaches maximum solubility in phase solubility study?

I am working on phase solubility studies between beta-cyclodextrin and drug? I used more concentration of beta-cyclodextrin till 30mM despite its maximum solubility of 16mM. Usually, above maximum...

30 October 2020 3,921 9 View

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30 October 2020 961 3 View

How to combine/add two Beta Distribution curves?

Hello, I am currently working on Beta Distribution, and I am using the distribution to model a knowledge/opinion into the software. The user adjusts the alpha and beta values of Beta...

12 September 2020 6,569 5 View

Why Beta-Galactosidase doesn't be sterilized?

The reason for sterilization through heat sterilization in Beta-Galactosidase production process is to remove other bacteria from the air. If this process is designed, the bacteria that produce...

08 September 2020 8,120 1 View