I am working with U87MG cell line and I got contamination in one of the flasks. Can anyone tell me what type of contamination is this?
I am trying to eliminate infection from my plates and therefore, gave UV treatment after addition of amino acid (used for better growth). Can you please suggest if UV destroys amino acids or not,...
02 July 2024 2,922 1 View
I have having issue with my DLS results
25 June 2024 2,218 1 View
I am working on an acetyltransferase that is highly unstable. Its pI is 6.65, and its molecular weight is around 18 kDa. The protein elutes at 1M imidazole and begins to precipitate immediately...
19 June 2024 2,590 0 View
Hii experts , i have synthesized a derivativ of biopolmer and zeta potential is a -1.2 mv,,can u tell me how it help me out to study my synthesized product ?
05 June 2024 4,720 3 View
Hi, I have been trying to extract Plant genomic DNA via CTAB method and I am getting a 260/280 ratio ranging between 1.3 to 1.6. Any idea how to modify the protocol for fabaceae family plant...
03 June 2024 7,722 6 View
Hi, I have few primers that are previously reported to amplify a conserved region. The ambiguity is that I want to be sure if they are correctly reported before ordering the same set of primers....
03 June 2024 4,167 3 View
Thermal water has Na-119 ppm Cl-469 ppm and SO4 -406 But it is not located anywhere near seawater area
01 May 2024 3,836 5 View
Is there a formula that I can use to calculate the power output using solar irradiance values?
01 May 2024 8,233 3 View
Dear Researchers What are the initial characterisations utilised to confirm the synthesis of MOF. Kindly suggest. Thank You
24 April 2024 1,425 2 View
Like, EPM or OFT.
18 April 2024 1,621 1 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...
26 July 2024 2,136 0 View
I suspect my cells are contaminated with mycoplasma. I fixed the cells with 4% PFA and stained them with DAPI. Below is the image I obtained. I don't observe the typical small, rounded DAPI foci...
11 July 2024 7,786 3 View
There are black dots that seem like contamination in my corneal endothelial cell cultures. Some of the cells appear unhealthy and are enlarged. The black dots are more prevalent around these...
11 July 2024 4,829 0 View
The question refer to leafy biomass decomposition using litterbag techniques.
11 July 2024 2,078 0 View
When Streptomyces undergoes conjugation transfer, it is always contaminated by a type of yellow Pseudomonas. What could be the reason for this?
07 July 2024 5,480 2 View
What is meant by cross-contamination and what causes fruits and vegetables to become contaminated during preparation and storage?
02 July 2024 9,204 0 View
We have been experiencing repeated what looks very much like fungal contaminations in several incubators (in the same lab). We have tried the following repeated times and yet the coating on all...
02 July 2024 6,375 2 View
Within our research lab we have access to PFOA in powder form and PFOS in 100microgram/L methanol solution. We want to make a PFAS contaminated water to test a PFAS treatment technology. Does...
01 July 2024 8,425 3 View