I have resuspended lyophilised Amyloid beta protein in Guandine HCL, how should I store it for long term use?

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Is it okay to grow bacteria in 30 for cloning? What is the lowest mass of Vecor I can take for cloning? what should be the optimum insert ratio?

I am using a vector that has repeat sequences, and using Mach1 competent cells. I heard that if the cells are not stable cells, then we shouldn't grow them in 37 to avoid recombination to happen...

26 June 2024 3,734 3 View

What is the the target displacement for a retrofitted model outside US conditions (preferably Indian conditions)?

Dear Members of ResearchGate, I hope you are doing well. I have been retrofitting using the ASCE 41 2017 standard. In it, I have two basic doubts. 1. The target displacement as per clause...

13 June 2024 2,773 0 View

What is the best book on seismic retrofitting reinforced concrete structures, preferably for Indian and US conditions (Recommendations please)?

Hello Everyone, I hope you are doing well. What is the best book on seismic retrofitting reinforced concrete structures, preferably for Indian and US conditions (Recommendations please)? Thank...

30 May 2024 9,200 9 View

Would you name some journals without APC to publish a small research methodology?

For publishing transformation related method I need name of some journals without costing us Article Processing Charges. Kindly help.

03 May 2024 1,896 1 View

Is there a way to map gene expression data from real-time PCR on gene trees?

I am trying to understand how the expression of paralogues changed over time. I have real-time PCR data and gene tree. I want to reconstruct the estimated gene expression level at each node. I...

14 March 2024 8,129 0 View

Please can anyone suggest me how to write a book chapter in Elsevier?

21 February 2024 3,672 2 View

How the 2% scientists in the world are selected by Stanford University?

For the past few years, Stanford University USA is coming up with a list of scientists, who are among the top 2% scientists in the world. I would like to know how the list is generated, by keeping...

26 January 2024 185 4 View

What is the best tool or platform for planning research works (Recommendations, please)?

Hi Everyone, I want to know some of the best tools or platforms for planning research works (PhD or/and Master's research). Till now I have been using MS Excel to schedule my work, but it is not...

15 January 2024 6,984 3 View

What are the values of electronic properties of Niobium Nitride (NbN)?

Can someone please help me by providing the values of electronic properties (bandgap,electron and hole density of states, dielectric permittivity and electron and hole mobilities) of Niobium...

02 January 2024 3,962 1 View

In Opensees, why reinforced concrete beam-column joint is assumed as a parallelogram?

Hi everyone. I wish you all a Merry Christmas and a Happy New Year ahead! I hope you are doing well. I have a question. In Opensees, why reinforced concrete beam-column joint is assumed as a...

25 December 2023 3,540 3 View

How to increase simulation box size?

We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...

08 August 2024 4,840 0 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,216 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,733 1 View

Recovery rate after peptide desalting using Sep-pack C18 1 cc Vac Cartridge?

Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?

31 July 2024 6,541 11 View

How can microbial engineering techniques be used to enhance the functionality and efficacy of probiotics ?

through genetic modification or metabolic engineering? Is it helpful, good for health ?

22 July 2024 7,811 4 View

Is it possible to conjugate an NHS ester to the N-terminus of a peptide ON RESIN?

So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...

18 July 2024 4,074 3 View

Does sucrose interfere with the BCA reagent in peptide concentration assays?

Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...

10 July 2024 6,828 1 View

What strains of C57 mice can induce rheumatoid arthritis?

Recently I looked up a literature《A Glucose-6-Phosphate Isomerase Peptide Induces T and BCell-Dependent Chronic Arthritis in C57BL/10 Mice Arthritis without Reactive Oxygen Species and...

09 July 2024 6,656 0 View