Is it justified to compare the free energy change of two reactions and conclude one is more favourable than the other?

Suppose we consider the binding of a ligand L to a metal M, M+L=ML and M+2L=ML2 . We compute the Gibbs free energy change for two reaction using DFT. But can we compare this two free energies to...

12 January 2021 5,893 4 View

How can i obtain electric displacement field in Piezoelectric bonded joint using Abaqus?

Mathematically, electric displacement is a vector quantity defined as {D}=([e]{E}+{P}), where {E} is local electric field, {P} is Polarization per unit volume and [e] is the third order tensor...

20 August 2020 4,789 1 View

How to calculate van der Waals radius theoretically?

I am trying to calculate van der Waals radius for silver atoms and ions. Could anyone explain the steps required to perform accurate calculations.

03 August 2020 2,574 3 View

How to align large plasmids (20Kb+) in Mac Vector?

I was trying to align 3 plasmids each around 20Kb in Mac Vector. I used the "Align to ref." tab followed by adding the sequences and then aligned them. However only a portion of each sequence is...

03 February 2020 2,635 1 View

How to do overlap PCR?

Hi I am trying to join two linear 1.2 kb fragments with overlaps. I read a protocol which says at least 1/2 of the PCR mix has to be the equimolar concentrations of the fragment. Why is so much...

30 May 2019 9,234 2 View

Is it possible to choose a site for setting a well using only remote sensing techniques and data ?

If I have only Microwave and Optical remotely sensed data, is it possible to choose the site for availability of ground water for set a pump or well so that it will reduce the chances of fail...

05 December 2018 3,372 13 View

How to create checkpoint file in molecular dynamics simulation?

Basically I want to save coordinates and momentum of atoms after n time step so that I can restart my calculation in case of an error or exceeding wall time. My MD code is in Fortran.

19 June 2018 8,262 5 View

What if Sodium pyruvate is added in excess in DMEM cell culture media?

What happens if we accidentally add more (5 ml of 200 mM) sodium pyruvate to 500 ml DMEM cell culture media that already contains 110 mg/ml sodium pyruvate in it (from company)? How will it...

29 November 2017 6,582 2 View

What are the different approaches for seperate few layer graphene from graphite?

31 August 2017 1,378 4 View

Availability of Sugarcane yellow leaf virus antibody/antiserum from any private firm?

Dear all...is there any information regarding availability of Sugarcane yellow leaf virus antibody/antiserum. previously i use to procure from AC, Diagnostics, USA but now they have discontinued...

24 January 2017 4,333 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View