ED50 of Meropenem in Mice against Klebsiella?

Somnath Chakravorty @Somnath-Chakravorty

19 June 2023 0 2K Report

I am looking for the ED50 of meropenem in mice against Klebsiella systemic/lung infection models. Is anybody aware of it?

Thank you.

Badges
Science topic

More Somnath Chakravorty's questions See All

Which method/ open-source software is the best for Microstructure modeling? Any relevant resources regarding microstructure modeling?

I am learning microstructure modeling and also looking for some resources and opensource software to start with.

05 April 2024 4,576 3 View

Is it correct to treat RAW264.7 mice macrophage cell line with recombinant Human Macrophage Migration Inhibitory Factor (MIF)?

Is it correct to treat RAW264.7 mice macrophage cell line with recombinant Human Macrophage Migration Inhibitory Factor (MIF). Will there be any species incompatibility related unwanted effect on...

12 November 2023 1,534 1 View

CMV and EF-1alpha promoter in same plasmid?

Hi I was wondering whether CMV and EF-1alpha promoters can be put in a single plasmid? Are there any compatibility issues between the two promoters? Thank you.

31 October 2023 9,919 2 View

What are the material characterzations that can be done on heterostructure based- materials and devices (like HEMTs, SBDs)?

Example: TEM reveals the layered stacks with different thicknesses

11 June 2023 7,653 3 View

Which softwares are better to draw schematic diagrams of beams, plates etc?

I want to know which software is used by authors to draw schematic diagrams of beams, plates etc. for there articles.

22 May 2023 7,631 6 View

Is there a publication that discusses expression of different glycosylation enzymes in different CHO cell lines?

Hi I am kind of looking to see if a comparative expression level analysis of the different Glycosylation enzymes of different CHO cell lines (DG44, DXB-11 and K1)is available in any publication. I...

12 April 2023 9,492 2 View

DTM is the only method to solve elastic support beam vibration problem??

Most of the available literature related to vibration of nanobeam with elastic support boundary condition, author use Differential Transform Method (DTM) to solve the governing differential...

19 March 2023 3,763 2 View

Site directed mutagenesis not working. Can anyone help?

I am trying to replace a 20 bp region with a different sequence of same lengthin a 10kb plasmid. I designed overlapping primers. However after PCR I am only getting the template. At first I...

13 February 2023 3,663 3 View

Why does relative energy of conformers change when dispersion corrections are included during DFT calculations?

During conformational analysis of 18crown6, it was observed that the conformer with S6 symmetry was having the lowest energy when dispersion correction was not included. However, upon addition of...

14 February 2022 1,493 3 View

THP-1 requires differentiation before Phagocytosis assay?

I am looking to study opsonophagocytosis using THP-1 cell line from ATCC. Does the cell line require differentiation before the phagocytosis assay?

28 October 2021 3,411 5 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,081 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,156 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,707 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 761 1 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,121 4 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,482 5 View