Is it correct to treat RAW264.7 mice macrophage cell line with recombinant Human Macrophage Migration Inhibitory Factor (MIF)?

13 November 2023 1 2K Report

Is it correct to treat RAW264.7 mice macrophage cell line with recombinant Human Macrophage Migration Inhibitory Factor (MIF). Will there be any species incompatibility related unwanted effect on the RAW264.7 cells?

Malcolm Nobre

Dear Dr. Somnath Mazumder

Treating RAW264.7 mice macrophage cell line with recombinant Human Macrophage Migration Inhibitory Factor (MIF) should not cause any problem. Using the protein from another species depends on the degree of homology between them. The mouse and human MIF sequences show 90% identity over 115 amino acids, which among the cytokines is the highest mouse to human homology.

You may want to refer to the paper attached below.

Article Purification, Bioactivity, and Secondary Structure Analysis ...

Many human cytokines will produce a good response in mouse cell lines, and many mouse proteins will show activity on human cells. So, go ahead and use the recombinant Human Macrophage Migration Inhibitory Factor (MIF) to treat RAW264.7 mice macrophage cell line.

Best.

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