Hi I was wondering whether CMV and EF-1alpha promoters can be put in a single plasmid? Are there any compatibility issues between the two promoters?
Thank you.
This a tandem promoter or separate parts of the plasmid?
Dear Dr. Chakravorty
You may use them as dual promoter namely, two promoters, one drives your gene-of-interest and the other drives one or more makers.
You may want to refer to the links below for more information.
https://www.cellomicstech.com/product/293/lentiviral-vector-cmv-ef1a-nls-rfp
https://cellecta.com/products/cdna-cloning-vector-with-cmv-promoter-cmv-mcs-ef1-selection?variant=17586335449178
https://fenicsbio.com/lentiviral-cloning-vector-cmv-ef1a-neo/
https://www.systembio.com/wp/wp-content/uploads/SBI_Brochure_Gene_Delivery_and_Expression.pdf
Best.
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