How to plot non-linear kinetics model for column sorption study?

Do I have to solver-add inn for its calculation?

10 December 2020 5,209 7 View

How to prevent calcium carbonate precipitation in chemically enhanced backwash dosing pipeline?

I am working on an experimental SWRO plant with Ultrafiltration module. The Chemical Enhanced Backwash cycle for the UF membrane constitutes a biocidal flushing (NaOCl) followed by low pH HCl...

09 September 2020 8,107 3 View

How to measure expression levels of a gene in two different cell lines?

If one needs to compare the expression levels of a particular gene experimentally (molecular method, not omics approach), can there be a reliable method to measure it? What kind of control can be...

02 December 2019 6,373 3 View

Some other person with similar name claim all of my research publication on research gate . how can I reclaim my Owen publication on this site?

Hi, I am bhushan mahajan, pursing my Phd from IICT Hyderabad India under Gudines of Dr. Ajay K Singh. sum of my co-author uploaded my publication detail on research gate, since i am not in...

15 November 2019 2,309 4 View

Have you used repertory grid interviews?

What have you used these for and where to learn about the various analysis that can be conducted using this method?

23 March 2019 8,369 2 View

Why do MPI-GPU based mdruns in GROMACS use less memory compared to MPI-OpenMP?

I am benchmarking my coarse-grained MD simulations with 30k beads. I noticed that MPI-GPU based mdruns used less memory and did not change much as I increased the number of nodes. Where as for...

15 July 2018 1,570 3 View

While doing Gene Set Enrichment Analysis, should one separate up- and down-regulated genes?

If we have a list of differential proteins from proteome data-sets, should we use all the differential genes together while running the analysis? Or, should those be run separately? Additionally,...

03 May 2018 7,296 3 View

What is the difference between HFD and db/db mice?

I am an undergraduate student currently using a HFD Mouse model to examine ER stress in various tissues implicated by type 2 diabetes. However I have noticed some papers use db/db mice ( C57BL/6J...

10 February 2018 3,166 2 View

How to detect a conserved region in a particular genus for which a genus specific primer can be designed?

05 May 2017 8,682 2 View

What are the factors/reasons for End splitting of a low carbon steel billet during hot rolling?

14 June 2016 3,468 1 View

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02 March 2021 3,060 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View