7 Questions 16 Answers 0 Followers
Questions related from Sonalika Mahajan
I have found 27 host proteins as interacting partner for the bait protein (Viral protein). out of them I have selected 3, all of them grew blue colonies on QDO?X/A. On colony lift filter assay,...
13 May 2015 5,732 6 View
I have expressed GST-tagged protein in BL21(DE3)pLysS cells but I am not able to get the protein in soluble form. Most of the protein is in inclusion body. The induction conditions used are 1mM...
02 May 2015 2,929 8 View
I want to identify the host proteins that interact with the non-coding RNA of virus. Is it possible to do the screening on the similar lines as done in protein-protein interactions using Y2H.
16 February 2015 2,125 4 View
I have estimated the virus titre to be 10 6.5 TCID50/50 µl. I want to know that how much virus I needed to add to infect 25cc flask at 1000 TCID50?
06 November 2014 1,750 2 View
I have screened a cDNA library of swine. I want to perform gene ontology on the obtained gene sequences so that a pie diagram representinbg the proportion of genes participating in various...
15 October 2014 4,168 10 View
I am trying to express the NSP gene of FMDv. I have cloned in pET-43.1a(+) vector and trying to express it in BL21(DE3)pLysS cells as Nus-tagged fusion protein. However, when I check the induced...
28 March 2014 9,058 1 View
I have found human proteins similar to my viral proteins. Now I am using this virus-similar human proteins to identify the interaction between my viral protein and human proteins. But I'm getting...
06 February 2014 2,072 7 View
