Sonalika Mahajan

6 Questions 13 Answers 0 Followers

Questions related from Sonalika Mahajan

Could somebody help me confirm protein interactions identified in yeast two-hybrid system?

I have found 27 host proteins as interacting partner for the bait protein (Viral protein). out of them I have selected 3, all of them grew blue colonies on QDO?X/A. On colony lift filter assay,...

13 May 2015 5,691 6 View

How can I express GST-tagged recombinant protein in soluble form and prevent the inclusion body formation?

I have expressed GST-tagged protein in BL21(DE3)pLysS cells but I am not able to get the protein in soluble form. Most of the protein is in inclusion body. The induction conditions used are 1mM...

02 May 2015 2,886 8 View

Where do I start screening for RNA-protein interactions?

I want to identify the host proteins that interact with the non-coding RNA of virus. Is it possible to do the screening on the similar lines as done in protein-protein interactions using Y2H.

16 February 2015 2,066 4 View

How to calcuate the dose of virus needed to infect 25cc flask at 1000 TCID50?

I have estimated the virus titre to be 10 6.5 TCID50/50 µl. I want to know that how much virus I needed to add to infect 25cc flask at 1000 TCID50?

06 November 2014 1,699 2 View

Can somebody please suggest some tools to perform Gene ontology t0 the third level using UniProt IDs of the genes ?

I have screened a cDNA library of swine. I want to perform gene ontology on the obtained gene sequences so that a pie diagram representinbg the proportion of genes participating in various...

15 October 2014 4,109 10 View

Has anyone expressed Nus tagged fusion protein in E.coli?

I am trying to express the NSP gene of FMDv. I have cloned in pET-43.1a(+) vector and trying to express it in BL21(DE3)pLysS cells as Nus-tagged fusion protein. However, when I check the induced...

28 March 2014 9,016 1 View