How to prevent calcium carbonate precipitation in chemically enhanced backwash dosing pipeline?

I am working on an experimental SWRO plant with Ultrafiltration module. The Chemical Enhanced Backwash cycle for the UF membrane constitutes a biocidal flushing (NaOCl) followed by low pH HCl...

09 September 2020 8,107 3 View

How to measure expression levels of a gene in two different cell lines?

If one needs to compare the expression levels of a particular gene experimentally (molecular method, not omics approach), can there be a reliable method to measure it? What kind of control can be...

02 December 2019 6,373 3 View

Some other person with similar name claim all of my research publication on research gate . how can I reclaim my Owen publication on this site?

Hi, I am bhushan mahajan, pursing my Phd from IICT Hyderabad India under Gudines of Dr. Ajay K Singh. sum of my co-author uploaded my publication detail on research gate, since i am not in...

15 November 2019 2,309 4 View

Have you used repertory grid interviews?

What have you used these for and where to learn about the various analysis that can be conducted using this method?

23 March 2019 8,369 2 View

Why do MPI-GPU based mdruns in GROMACS use less memory compared to MPI-OpenMP?

I am benchmarking my coarse-grained MD simulations with 30k beads. I noticed that MPI-GPU based mdruns used less memory and did not change much as I increased the number of nodes. Where as for...

15 July 2018 1,570 3 View

Is there a list of UGC approved community medicine or public health journals in India?

04 May 2018 5,865 5 View

While doing Gene Set Enrichment Analysis, should one separate up- and down-regulated genes?

If we have a list of differential proteins from proteome data-sets, should we use all the differential genes together while running the analysis? Or, should those be run separately? Additionally,...

03 May 2018 7,296 3 View

What is the difference between HFD and db/db mice?

I am an undergraduate student currently using a HFD Mouse model to examine ER stress in various tissues implicated by type 2 diabetes. However I have noticed some papers use db/db mice ( C57BL/6J...

10 February 2018 3,166 2 View

What are the factors/reasons for End splitting of a low carbon steel billet during hot rolling?

14 June 2016 3,468 1 View

In Researcher , what characteristics do you think the main ?

21 March 2016 1,957 14 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View