Rajiv Mohan

3 Questions 8 Answers 0 Followers

Questions related from Rajiv Mohan

Does the virus titer of stored enveloped virus (-80C) in time declines (IU/mL) at a different rate than GC/mL?

I know that virus titers of stored viruses goes down in time due to damage. So the infectious units per mL (IU/mL) will decline. What I could imagine is that although the IU/mL goes down, the...

11 June 2020 755 0 View

Is it possible to do a lentiviral infection/transduction on trypsinized cells still in cell suspension while plating them in Exp plate?

I have a lentivirus (VSV-G) containing an enhancer and general promoter coupled to a fluorescent protein-encoding gene (mCherry). The vector is used as a tool to identify factors able to stimulate...

21 January 2020 3,333 3 View

Do you need a higher sequencing depth (> 30*10^6) with low amount RNA samples (500 pg)?

Approximately 500pg to 1.5 ng Total RNA was obtained using the Picopure RNA isolation kit with an on column DNAse treatment. Thereafter, the Nugen ovation RNAseq V2 amplification kit was used to...

14 June 2017 7,943 1 View