A previous colleague homogenized brain samples and stored the supernatant at -80C. According to their lab notes, the homogenized samples were centrifuged at 13,000rpm, for 15 minutes at 4C. The supernatant was removed and stored in a separate tube, while the pellet was discarded. I've been asked to look at some synaptic markers (PSD-95, f-actin, etc.) via Western Blot using this tissue, however, I'm concerned about the success of the blots due to how they were initially homogenized/processed. From what I've been reading, in order to accurately target synaptic proteins, the homogenized tissue has to undergo multiple rounds of supernatant removal, pellet rehydration, and centrifuge at various speeds/times.

Since the previous tissue was only processed under the conditions listed above, is it possible to salvage these samples to investigate synaptic markers?

This is old tissue to a particular project, so we cannot go back and gather more. The colleague who initially processed this wasn't intending on looking at synaptic proteins, so I can't blame them for doing it this way. Does anyone have any suggestions?